Protein Therapeutics Scientist/Sr Scientist Protein Expression
Zoetis is a global animal health company dedicated to supporting customers and their businesses in ever better ways. Building on 60 years of experience, we deliver quality medicines and vaccines, complemented by diagnostic products and genetic tests and supported by a range of services. We are working every day to better understand and address the real-world challenges faced by those who raise and care for animals in ways they find truly relevant.Our name, Zoetis (z-EH-tis), has its root in zo, familiar in words such as zoo and zoology and derived from zoetic, meaning “pertaining to life.” It signals our company”s dedication to supporting the veterinarians and livestock producers everywhere who raise and care for the farm and companion animals on which we all depend on.Zoetis is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status or any other protected classification. Disabled individuals are given an equal opportunity to use our online application system. Zoetis also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as employment eligibility verification requirements of the Immigration and Nationality Act. All applicants must possess or obtain authorization to work in the US for Zoetis. Zoetis retains sole and exclusive discretion to pursue sponsorship for the acquisition or maintenance of nonimmigrant status and employment eligibility, considering factors such as availability of qualified US workers. Individuals requiring sponsorship must disclose this fact.Please note that Zoetis seeks information related to job applications from candidates for jobs in the U.S. solely via the following: (1) our company website at www.Zoetis.com/careers site, or (2) via email to/from addresses using only the Zoetis domain of “@zoetis.com”. In addition, Zoetis does not use Google Hangout for any recruitment related activities. Any solicitation or request for information related to job applications with Zoetis via any other means and/or utilizing email addresses with any other domain should be disregarded. In addition, Zoetis will never ask candidates to make any type of personal financial investment related to gaining employment with Zoetis.The Protein Therapeutics group within Global Therapeutics Research at Zoetis has the mission and responsibility for identifying and delivering novel large and small molecule therapeutics for a diverse array of indications. We are seeking a talented and experienced bench scientist with strong molecular and cell biology experience to join our efforts in mammalian cell line engineering and protein expression. The ideal candidate will have demonstrated an ability to work independently at the bench with a variety of vector platforms, cellular delivery methods, cell growth and expression systems using both transient and stable modalities. The colleague will be expected to design, explore and successfully integrate new cell engineering techniques and automation/high-throughput technologies into a research scale protein production workflow to support therapeutic candidate evaluations by discovery teams. In addition, the colleague will assist in supporting day-to-day activities for routine production of biologicals. Position Responsibilities:*Mammalian expression of recombinant gene constructs in transient and stable formats for candidate molecule screening and/or production*Assist with production of biological candidates for Discovery Research screening*Improve and optimize protein expression and other desired properties of host cells through targeted genetic modifications and other molecular engineering approaches*Maintain state of the art in protein expression through continually improving/enhancing internal capabilities, including the development of automated and high-throughput platforms, and by leveraging external technology*Perform small to mid-scale protein production runs for protein purification and carry out expression characterization such as gel electrophoresis, western-blot and FACS*Collaborate with the Biopharmaceutical Development Group to evaluate new production platforms and to ensure suitability of cell line platform modifications for development and manufacturing use*Participate in technology evaluations of alternate mammalian expression platforms*Prepare precise technical reports summaries, protocols, quantitative analyses, and maintain appropriate documentation*Maintain high scientific rigor and attention to detail in designing and testing hypotheses and analyzing data. Education and Experience:*MS or PhD in Cell Biology, Molecular Biology, or related field, with a minimum of 3 years laboratory experience with cell culture, preferably in an industry environment*Extensive recombinant protein expression experience in mammalian cell systems (especially HEK/CHO) is required*Experience in the generation and functional assessment of recombinant cell lines expressing various target proteins (GPCRs, ion channels, etc)*Training and demonstrated proficiency in cell biology and aseptic cell culture with a wide variety of cell types*Working knowledge in molecular biology, cloning and protein biochemistry* Demonstrated proficiency with protein expression analysis techniques (e.g. gel electrophoresis, Western Blot, flow cytometry)*Hands-on experience with WAVE reactors desired, and experience with tangential flow filtration would be a plus* Knowledge of industrial technology in CHO cell culture, and working knowledge in cell physiology is an advantage*Additional skills such as viral transduction, bioengineering, stable cell line generation, gene-editing, high-throughput molecular biology, single cell profiling, and familiarity with CMC regulatory requirements are all highly desirable.*Strong scientific curiosity, ability to generate and consider alternative approaches for technological challenges, strong troubleshooting ability.*Scientifically rigorous, highly organized, able to manage multiple simultaneous projects and significant attention to detailApply for this job.